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Low-dose X-NP-DOX inhibits cell growth, migration, invasion, and induces cell apoptosis in vitro . The CCK8 assay, wound-healing assay, and <t>Transwell</t> chambers assay were used to analyze cell growth, migration, and cell invasion; cell apoptosis was measured by Hoechst 33258 staining or Annexin V staining. The CCK8 assay, wound-healing assay, transwell chambers assay, and apoptosis staining was performed as described in Materials and Methods section. (A) The antitumor activity of X-NP-DOX in MCF-7 by CCK8 assays. Under the treatment of X-NP-DOX or free DOX, the proliferation of MCF-7 cells was markedly inhibited compared with the PBS group ( p < .05). (B) The transwell system assay illustrated that the invasiveness of MCF-7 was inhibited by low-dose X-NP-DOX (magnification, 400×). The transwell system assay showed that the percentage of MCF-7 invasiveness treated with X-NP-DOX was significantly lower than that of PBS-treated cells ( p < .05). (C) The wound-healing assay showed that the number of migrating cells treated with low-dose X-NP-DOX was significantly less compared to free DOX and PBS groups. (D, E) Hoechst 33258 staining (D) and Annexin V-FITC staining (E) results showed that early apoptotic cells were observed in low-dose X-NP-DOX-treated group.
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Low-dose X-NP-DOX inhibits cell growth, migration, invasion, and induces cell apoptosis in vitro . The CCK8 assay, wound-healing assay, and <t>Transwell</t> chambers assay were used to analyze cell growth, migration, and cell invasion; cell apoptosis was measured by Hoechst 33258 staining or Annexin V staining. The CCK8 assay, wound-healing assay, transwell chambers assay, and apoptosis staining was performed as described in Materials and Methods section. (A) The antitumor activity of X-NP-DOX in MCF-7 by CCK8 assays. Under the treatment of X-NP-DOX or free DOX, the proliferation of MCF-7 cells was markedly inhibited compared with the PBS group ( p < .05). (B) The transwell system assay illustrated that the invasiveness of MCF-7 was inhibited by low-dose X-NP-DOX (magnification, 400×). The transwell system assay showed that the percentage of MCF-7 invasiveness treated with X-NP-DOX was significantly lower than that of PBS-treated cells ( p < .05). (C) The wound-healing assay showed that the number of migrating cells treated with low-dose X-NP-DOX was significantly less compared to free DOX and PBS groups. (D, E) Hoechst 33258 staining (D) and Annexin V-FITC staining (E) results showed that early apoptotic cells were observed in low-dose X-NP-DOX-treated group.
Cell Matrix Bd Matrigeltm Matrix, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bd matrigeltm basement membrane matrix  (Biocoat)
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Biocoat bd matrigeltm basement membrane matrix
Low-dose X-NP-DOX inhibits cell growth, migration, invasion, and induces cell apoptosis in vitro . The CCK8 assay, wound-healing assay, and <t>Transwell</t> chambers assay were used to analyze cell growth, migration, and cell invasion; cell apoptosis was measured by Hoechst 33258 staining or Annexin V staining. The CCK8 assay, wound-healing assay, transwell chambers assay, and apoptosis staining was performed as described in Materials and Methods section. (A) The antitumor activity of X-NP-DOX in MCF-7 by CCK8 assays. Under the treatment of X-NP-DOX or free DOX, the proliferation of MCF-7 cells was markedly inhibited compared with the PBS group ( p < .05). (B) The transwell system assay illustrated that the invasiveness of MCF-7 was inhibited by low-dose X-NP-DOX (magnification, 400×). The transwell system assay showed that the percentage of MCF-7 invasiveness treated with X-NP-DOX was significantly lower than that of PBS-treated cells ( p < .05). (C) The wound-healing assay showed that the number of migrating cells treated with low-dose X-NP-DOX was significantly less compared to free DOX and PBS groups. (D, E) Hoechst 33258 staining (D) and Annexin V-FITC staining (E) results showed that early apoptotic cells were observed in low-dose X-NP-DOX-treated group.
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Low-dose X-NP-DOX inhibits cell growth, migration, invasion, and induces cell apoptosis in vitro . The CCK8 assay, wound-healing assay, and Transwell chambers assay were used to analyze cell growth, migration, and cell invasion; cell apoptosis was measured by Hoechst 33258 staining or Annexin V staining. The CCK8 assay, wound-healing assay, transwell chambers assay, and apoptosis staining was performed as described in Materials and Methods section. (A) The antitumor activity of X-NP-DOX in MCF-7 by CCK8 assays. Under the treatment of X-NP-DOX or free DOX, the proliferation of MCF-7 cells was markedly inhibited compared with the PBS group ( p < .05). (B) The transwell system assay illustrated that the invasiveness of MCF-7 was inhibited by low-dose X-NP-DOX (magnification, 400×). The transwell system assay showed that the percentage of MCF-7 invasiveness treated with X-NP-DOX was significantly lower than that of PBS-treated cells ( p < .05). (C) The wound-healing assay showed that the number of migrating cells treated with low-dose X-NP-DOX was significantly less compared to free DOX and PBS groups. (D, E) Hoechst 33258 staining (D) and Annexin V-FITC staining (E) results showed that early apoptotic cells were observed in low-dose X-NP-DOX-treated group.

Journal: Drug Delivery

Article Title: Enhanced chemotherapeutic efficacy of the low-dose doxorubicin in breast cancer via nanoparticle delivery system crosslinked hyaluronic acid

doi: 10.1080/10717544.2018.1507057

Figure Lengend Snippet: Low-dose X-NP-DOX inhibits cell growth, migration, invasion, and induces cell apoptosis in vitro . The CCK8 assay, wound-healing assay, and Transwell chambers assay were used to analyze cell growth, migration, and cell invasion; cell apoptosis was measured by Hoechst 33258 staining or Annexin V staining. The CCK8 assay, wound-healing assay, transwell chambers assay, and apoptosis staining was performed as described in Materials and Methods section. (A) The antitumor activity of X-NP-DOX in MCF-7 by CCK8 assays. Under the treatment of X-NP-DOX or free DOX, the proliferation of MCF-7 cells was markedly inhibited compared with the PBS group ( p < .05). (B) The transwell system assay illustrated that the invasiveness of MCF-7 was inhibited by low-dose X-NP-DOX (magnification, 400×). The transwell system assay showed that the percentage of MCF-7 invasiveness treated with X-NP-DOX was significantly lower than that of PBS-treated cells ( p < .05). (C) The wound-healing assay showed that the number of migrating cells treated with low-dose X-NP-DOX was significantly less compared to free DOX and PBS groups. (D, E) Hoechst 33258 staining (D) and Annexin V-FITC staining (E) results showed that early apoptotic cells were observed in low-dose X-NP-DOX-treated group.

Article Snippet: The cell invasion assay was performed in Transwell chambers coated with BD MatrigelTM matrix (3 mg/mL) (BD Biosciences, Becton Dickson Labware, Franklin Lakes, NJ).

Techniques: Migration, In Vitro, CCK-8 Assay, Wound Healing Assay, Staining, Activity Assay